CRISPR-Cas: a Laboratory Manual
馆藏地：主馆A400阅览室
索书号：Q75 C932C 2016
著者：Jennifer Doudna
出版社：Cold Spring Harbor Laboratory Press
出版年：2016
简介：Featuring contributions from leading figures in the CRISPR-Cas field, this book presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells(e.g., human pluripotent stem cells). Strategies for optimizing CRISPR-Cas in each system, especially for minimizing off-target effects, are also provided.
Modern Prometheus: Editing the Human Genome with Crispr-Cas9
电子书全文
https://www.cambridge.org/core/books/modern-prometheus/663B2AD6EE426536FD8A9DB2DAD6614C
著者：Jim Kozubek
出版社：Cambridge University Press
出版年：2016
简介：Kozubek weaves together the fascinating stories of many of the scientists involved in the development of gene editing technology. Along the way, he demystifies how the technology really works and provides vivid and thought-provoking reflections on the continuing ethical debate. Ultimately, Kozubek places the debate in its historical and scientific context to consider both what drives scientific discovery and the implications of the 'commodification' of life.
Genome Editing
电子书全文
https://link.springer.com/book/10.1007%2F978-3-319-34148-4
著者：Kursad Turksen
出版社：Springer Press
出版年：2016
简介：This book explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. This book describes latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others.
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编辑│ 吴秋林
来源│ 生命医药学科服务
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